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Amaxa amaxa 4d nucleofector
Amaxa 4d Nucleofector, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+nucleofector/4d+amaxa+nucleofector/pmc13253082-82-0-0
Average 86 stars, based on 1 article reviews
amaxa 4d nucleofector - by Bioz Stars, 2026-09
86/100 stars

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Knock-In:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. FIG. 3D shows ZFP-TFs 30640 and 30657 (fused to the KRAB repression domain of KOX1) can repress the knock-in Htt allele (CAG111) in immortalized striatal cells derived from the Hdh(Q111/Q7) knock-in mice, demonstrating the ZFPs such as 30640, which drives CAG repeat length-dependent repression of luciferase reporters, can also repress expression from an endogenous Htt allele that has expanded CAG repeat. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and transfected into Hdh(Q111/Q7) cells at indicated doses using Amaxa nucleofector. ..

Derivative Assay:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. FIG. 3D shows ZFP-TFs 30640 and 30657 (fused to the KRAB repression domain of KOX1) can repress the knock-in Htt allele (CAG111) in immortalized striatal cells derived from the Hdh(Q111/Q7) knock-in mice, demonstrating the ZFPs such as 30640, which drives CAG repeat length-dependent repression of luciferase reporters, can also repress expression from an endogenous Htt allele that has expanded CAG repeat. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and transfected into Hdh(Q111/Q7) cells at indicated doses using Amaxa nucleofector. ..

Luciferase:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. FIG. 3D shows ZFP-TFs 30640 and 30657 (fused to the KRAB repression domain of KOX1) can repress the knock-in Htt allele (CAG111) in immortalized striatal cells derived from the Hdh(Q111/Q7) knock-in mice, demonstrating the ZFPs such as 30640, which drives CAG repeat length-dependent repression of luciferase reporters, can also repress expression from an endogenous Htt allele that has expanded CAG repeat. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and transfected into Hdh(Q111/Q7) cells at indicated doses using Amaxa nucleofector. ..

Expressing:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. FIG. 3D shows ZFP-TFs 30640 and 30657 (fused to the KRAB repression domain of KOX1) can repress the knock-in Htt allele (CAG111) in immortalized striatal cells derived from the Hdh(Q111/Q7) knock-in mice, demonstrating the ZFPs such as 30640, which drives CAG repeat length-dependent repression of luciferase reporters, can also repress expression from an endogenous Htt allele that has expanded CAG repeat. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and transfected into Hdh(Q111/Q7) cells at indicated doses using Amaxa nucleofector. ..

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. Different amounts of ZFP mRNA were transfected using Amaxa nucleofector as indicated, expression of the mutant Htt (right bar), wild type Htt (middle bar) and total Htt (both alleles, left bar) were measured as described above at 24 hours after transfection. ..

Generated:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. FIG. 3D shows ZFP-TFs 30640 and 30657 (fused to the KRAB repression domain of KOX1) can repress the knock-in Htt allele (CAG111) in immortalized striatal cells derived from the Hdh(Q111/Q7) knock-in mice, demonstrating the ZFPs such as 30640, which drives CAG repeat length-dependent repression of luciferase reporters, can also repress expression from an endogenous Htt allele that has expanded CAG repeat. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and transfected into Hdh(Q111/Q7) cells at indicated doses using Amaxa nucleofector. ..

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. See, FIGS. 2D and 2E. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and 0.1, 0.5 or 2 ug of these mRNAs were transfected using Amaxa nucleofector as described above. ..

Transfection:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. FIG. 3D shows ZFP-TFs 30640 and 30657 (fused to the KRAB repression domain of KOX1) can repress the knock-in Htt allele (CAG111) in immortalized striatal cells derived from the Hdh(Q111/Q7) knock-in mice, demonstrating the ZFPs such as 30640, which drives CAG repeat length-dependent repression of luciferase reporters, can also repress expression from an endogenous Htt allele that has expanded CAG repeat. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and transfected into Hdh(Q111/Q7) cells at indicated doses using Amaxa nucleofector. ..

Article Title: KREPA6 functions in RNA editing catalytic complex structural organization and gRNA utilization in Trypanosoma brucei
Article Snippet: .. The resulting construct (BSD-HSVTK with homology arms) was transfected into BF SM427 cells using the Amaxa nucleofector as previously described and transfectants were selected with 4 μg/ml blasticidin to isolate the BF KREPA6-SKO cell line [ ]. ..

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. Human HEK293 cells (Graham, et al. (1977) J Gen Virol 36:59-74) were cultured in DMEM supplemented with 10% FBS and 1e5 cells were transfected with 1 μg of plasmid DNA encoding indicated ZFP-KOX fusions by Amaxa Nucleofector® following the manufacturer's instructions. .. Transfected cells were incubated for 2 days, and the levels of endogenous human Huntingtin (Htt) and normalization control beta-actin (ACTB) were analyzed by real-time PCR using Hs00918176_m1 and 4352935E primers and probes (Applied Biosystems), respectively, according to standard protocols.

Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
Article Snippet: .. The plasmid was transfected into BEAS-2B MR1 KO tet MR1-GFP clonal cell lines D4 and D6 using an Amaxa Nucleofector with Nucleofection Kit T (Lonza) according to the manufacturer’s instructions. ..

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. See, FIGS. 2D and 2E. mRNA for indicated ZFPs were generated using the mMessage mMachine kit (Ambion), and 0.1, 0.5 or 2 ug of these mRNAs were transfected using Amaxa nucleofector as described above. ..

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. Different amounts of ZFP mRNA were transfected using Amaxa nucleofector as indicated, expression of the mutant Htt (right bar), wild type Htt (middle bar) and total Htt (both alleles, left bar) were measured as described above at 24 hours after transfection. ..

Construct:

Article Title: KREPA6 functions in RNA editing catalytic complex structural organization and gRNA utilization in Trypanosoma brucei
Article Snippet: .. The resulting construct (BSD-HSVTK with homology arms) was transfected into BF SM427 cells using the Amaxa nucleofector as previously described and transfectants were selected with 4 μg/ml blasticidin to isolate the BF KREPA6-SKO cell line [ ]. ..

Article Title: KREPA6 functions in RNA editing catalytic complex structural organization and gRNA utilization in Trypanosoma brucei.
Article Snippet: .. The esulting construct (BSD-HSVTK with homology arms) was ransfected into BF SM427 cells using the Amaxa nucleofector s previously described and transfectants were selected with 4 g/ml blasticidin to isolate the BF KREP A6-SK O cell line [ 48 ]. ..

Cell Culture:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. Human HEK293 cells (Graham, et al. (1977) J Gen Virol 36:59-74) were cultured in DMEM supplemented with 10% FBS and 1e5 cells were transfected with 1 μg of plasmid DNA encoding indicated ZFP-KOX fusions by Amaxa Nucleofector® following the manufacturer's instructions. .. Transfected cells were incubated for 2 days, and the levels of endogenous human Huntingtin (Htt) and normalization control beta-actin (ACTB) were analyzed by real-time PCR using Hs00918176_m1 and 4352935E primers and probes (Applied Biosystems), respectively, according to standard protocols.

Plasmid Preparation:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. Human HEK293 cells (Graham, et al. (1977) J Gen Virol 36:59-74) were cultured in DMEM supplemented with 10% FBS and 1e5 cells were transfected with 1 μg of plasmid DNA encoding indicated ZFP-KOX fusions by Amaxa Nucleofector® following the manufacturer's instructions. .. Transfected cells were incubated for 2 days, and the levels of endogenous human Huntingtin (Htt) and normalization control beta-actin (ACTB) were analyzed by real-time PCR using Hs00918176_m1 and 4352935E primers and probes (Applied Biosystems), respectively, according to standard protocols.

Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
Article Snippet: .. The plasmid was transfected into BEAS-2B MR1 KO tet MR1-GFP clonal cell lines D4 and D6 using an Amaxa Nucleofector with Nucleofection Kit T (Lonza) according to the manufacturer’s instructions. ..

Mutagenesis:

Article Title: Methods and compositions for treating Huntington's Disease
Article Snippet: .. Different amounts of ZFP mRNA were transfected using Amaxa nucleofector as indicated, expression of the mutant Htt (right bar), wild type Htt (middle bar) and total Htt (both alleles, left bar) were measured as described above at 24 hours after transfection. ..



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